3d max 2010 Search Results


3d reconstructions  (Oxford Instruments)
99
Oxford Instruments 3d reconstructions
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
3d Reconstructions, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3d studio max 2010  (Autodesk Inc)
90
Autodesk Inc 3d studio max 2010
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
3d Studio Max 2010, supplied by Autodesk Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3d studio max 2010/product/Autodesk Inc
Average 90 stars, based on 1 article reviews
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autodesk character generator  (Autodesk Inc)
90
Autodesk Inc autodesk character generator
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
Autodesk Character Generator, supplied by Autodesk Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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cubic splines in software autodesk 3ds max (version 2010)  (Autodesk Inc)
90
Autodesk Inc cubic splines in software autodesk 3ds max (version 2010)
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
Cubic Splines In Software Autodesk 3ds Max (Version 2010), supplied by Autodesk Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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volume mesh generation program  (Autodesk Inc)
90
Autodesk Inc volume mesh generation program
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
Volume Mesh Generation Program, supplied by Autodesk Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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medium 154  (Thermo Fisher)
90
Thermo Fisher medium 154
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
Medium 154, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/medium 154/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
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3d studio max 2011  (Autodesk Inc)
90
Autodesk Inc 3d studio max 2011
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
3d Studio Max 2011, supplied by Autodesk Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3d studio max 2011/product/Autodesk Inc
Average 90 stars, based on 1 article reviews
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3dvia virtools version 4.1  (Dassault Systemes)
90
Dassault Systemes 3dvia virtools version 4.1
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
3dvia Virtools Version 4.1, supplied by Dassault Systemes, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3dvia virtools version 4.1/product/Dassault Systemes
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lsqnonlin in matlab  (MathWorks Inc)
90
MathWorks Inc lsqnonlin in matlab
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
Lsqnonlin In Matlab, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3.0 t mri system  (Philips Healthcare)
90
Philips Healthcare 3.0 t mri system
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
3.0 T Mri System, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3.0 t mri system/product/Philips Healthcare
Average 90 stars, based on 1 article reviews
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topspin 4 1 4 bruker  (Bruker Corporation)
96
Bruker Corporation topspin 4 1 4 bruker
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
Topspin 4 1 4 Bruker, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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agility® system  (DYNEX tech)
90
DYNEX tech agility® system
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
Agility® System, supplied by DYNEX tech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged using confocal microscopy (A). (B-C) Representative max projections of 3D reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)

Journal: Glia

Article Title: A novel myelin protein zero transgenic zebrafish designed for rapid readout of in vivo myelination.

doi: 10.1002/glia.23559

Figure Lengend Snippet: Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged using confocal microscopy (A). (B-C) Representative max projections of 3D reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)

Article Snippet: Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged using confocal microscopy (A). (B-C) Representative max projections of 3D reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****) qPCR analysis for common myelin mRNAs showed relatively comparable responses to the drugs ( ). mpz , mbp and 36K transcripts, which are the most well characterized myelin-specific transcripts in zebrafish ( Q. Bai, M. Sun, D. B. Stolz, & E. A. Burton, 2011b ; C. E. Buckley et al., 2010 ; Morris et al., 2004 ) were quantified, and the changes in EGFP intensity generally mirrored changes in mpz mRNA expression as assayed by qPCR (Relative changes [RQ] for GC1: 2.29 fold increase, p<0.0166; Way200070: 1.75 fold increase, p<0.0079; rapamycin: 0.40 fold vs DMSO, p<0.0182 and Ag1478: 0.77 fold decrease, p<0.402; ).

Techniques: Incubation, Confocal Microscopy, Software, Fluorescence

Average number of dorsal oligodendrocyte lineage cells (olig2+/sox10+) was quantified from 3D reconstructions of live images of the lateral spinal cord of Tg(olig2:EGFP; sox10:T-RFP) larvae (A) or myelinating oligodendrocytes (olig2+/mbp+) from Tg(olig2:EGFP; mbp1a:T-RFP) larvae (B) following 10nM GC1 or 20μM rapamycin treatment (2–4dpf). (C-E) Representative images of individual scatter labeled oligodendrocytes and quantitative analysis of internode length (F), number of internodes (G) and total internode length generated per cell (H) following 10nM GC1 or 20μM rapamycin treatment (2–4dpf). (scale bar =25μM, statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)

Journal: Glia

Article Title: A novel myelin protein zero transgenic zebrafish designed for rapid readout of in vivo myelination.

doi: 10.1002/glia.23559

Figure Lengend Snippet: Average number of dorsal oligodendrocyte lineage cells (olig2+/sox10+) was quantified from 3D reconstructions of live images of the lateral spinal cord of Tg(olig2:EGFP; sox10:T-RFP) larvae (A) or myelinating oligodendrocytes (olig2+/mbp+) from Tg(olig2:EGFP; mbp1a:T-RFP) larvae (B) following 10nM GC1 or 20μM rapamycin treatment (2–4dpf). (C-E) Representative images of individual scatter labeled oligodendrocytes and quantitative analysis of internode length (F), number of internodes (G) and total internode length generated per cell (H) following 10nM GC1 or 20μM rapamycin treatment (2–4dpf). (scale bar =25μM, statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)

Article Snippet: Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged using confocal microscopy (A). (B-C) Representative max projections of 3D reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****) qPCR analysis for common myelin mRNAs showed relatively comparable responses to the drugs ( ). mpz , mbp and 36K transcripts, which are the most well characterized myelin-specific transcripts in zebrafish ( Q. Bai, M. Sun, D. B. Stolz, & E. A. Burton, 2011b ; C. E. Buckley et al., 2010 ; Morris et al., 2004 ) were quantified, and the changes in EGFP intensity generally mirrored changes in mpz mRNA expression as assayed by qPCR (Relative changes [RQ] for GC1: 2.29 fold increase, p<0.0166; Way200070: 1.75 fold increase, p<0.0079; rapamycin: 0.40 fold vs DMSO, p<0.0182 and Ag1478: 0.77 fold decrease, p<0.402; ).

Techniques: Labeling, Generated